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Another interesting phenomenon is that the staining of signaling molecules on mES cells was heterogeneous, the fluorescence strength of varied between Oct4 positive cells.
The fluorescence strength was analysed using scion image (Scion Cooperation, Frederick, Maryland, USA).
Let us compare this result with the SNR for a conventional TPEF microscope that uses a power P0 to image a fluorescence strength X.
Despite the simplifications used in our model, our theoretical predictions of fluorescence strength fit well to our experimental results for both imaging modalities.
In addition, the fluorescence intensity in the HepG2 cells treated with Asp-DOX was stronger than that in HepG2 cells treated with DOX (Fig. 3a), whereas the fluorescence strength in H1299 cells (Fig. 3b) was reversed compared to HepG2.
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The fluorescence intensity was monitored until the signal strength became indistinguishable from the background.
Differences in the overall strength of the decay in fluorescence intensity indicate slightly different binding geometry for the four GAGs.
The images acquired were tested for uniformity of illumination and fluorescence emission strength.
The consensus binding sequence was determined based on the fluorescence signal strength according to previously described methods (Jung et al. 2012; Kim et al. 2009).
As shown in Fig. 10a, after CdSe/ZnS QDs-labeled MSCs were injected into mice for 1 h, MSCs accumulated in the lungs and abdominal cavity, and the fluorescence signal strength had no significant difference between the normal control group and diabetes group.
Fluorescence signal strength depends on dye loading, tissue integrity/viability, and underlying structures (e.g., cleavage planes between cell layers).
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