Sentence examples for fluorescence sequence from inspiring English sources

Input to CellMAP is any high contrast, time-lapse fluorescence sequence of a single cell whose boundary lies entirely within every frame of the sequence (Fig. 1b).

The result is a one-dimensional fluorescence sequence.

Automated fluorescence sequencing was performed on an Applied Biosystems 310 DNA sequencer (PE Applied Biosystem).

Q-values can be calculated from standard fluorescence sequencing data (capillary electropherograms) and the degeneracy predicted from an early stage of library construction (pooled plasmids from the initial transformation) closely matched that observed after ca. 1000 library members were sequenced.

Verification of correct sequence and frame was obtained from forward and reverse automated fluorescence sequencing.

Automated fluorescence sequencing was carried out with a BigDye Terminator Ready Reaction Kit (Applied Biosystems) on a 3130XL Genetic Analyser (Applied Biosystems) according to manufacturer's protocols.

Fluorescence sequencing of exons 1 5, 7, 9 13 (including intronic boundaries) of MAPT gene was performed.

An additional 15 samples were sequenced for coding regions of H3F3A and HIST1H3B/C using Sanger fluorescence sequencing.

It utilizes the technology of DNA megacloning in combination with non-gel-based signature fluorescence sequencing to generate very large numbers of short read-length sequences (9).

All of these libraries were screened "blind", that is, not sequenced prior to screening, and only those clones showing improved properties were subsequently examined by fluorescence sequencing.

All pooled libraries were analyzed quantitatively after the initial transformation step by fluorescence sequencing to assess diversity at the randomized positions (Table 1).