Sentence examples for fluorescence screen from inspiring English sources

The tryptophan fluorescence screen does not require development of a fluorescent or radio-labeled ligand, has a high signal- to-noise ratio, and could be easily automated for a high-throughput format.

The current density can be measured from the fluorescence screen to convert it to the dose rate on the specimen.

To discriminate between germline and embryonic chemically induced defects, we followed the fluorescence screen with two assays: a) a reporter-based germline apoptosis assay (Zhou et al. 2001), and b) DAPI-staining of the germline nuclei.

Partial fluorescence yield data were recorded in scanning mode by a grating with line density 1200 lines mm−1 and radius 7.5 m dispersing the emitted photon energy from the sample,[ 14] and subsequently a detector consisting of a charge-coupled device, fluorescence screen and microchannel plate stack collecting the amplified signal.

Here, we address the negative effects of protein folding on fluorescence screening with three alternative methods that utilize fluorescent proteins to screen for successful gene integration.

Testing of this plasmid vector for enhanced fluorescence screening was conducted by inserting the PCR fragment encoding the fluorescent protein hcRed (between MCS1 and MCS2) [10].

Since the most N-terminal protein has the best opportunity for proper protein-folding and proper folding of our reporter fluorescent protein is imperative for efficient screening, another method to enhance on-plate fluorescence screening is creating gene inserts such that its integration will reconstitute a truncated N-terminal fluorescent protein.

Fluorescence screening of NK and target cells confined in microwells greatly facilitated both acquiring and analysis of the data.

Enhanced fluorescence screening was also seen when other gene of varying length and folding efficiency was inserted into the N-terminally situated CaM plasmid expression vector (Fig. S1).

Fluorescence screening of gene insertion on bacterial culture plates can be performed using the pCfvtx plasmid from Truong's cassette methodology because the successful insertion creates a C-terminal fusion of the gene with Venus (yellow fluorescent protein mutant)[6].

The dye used was 8 µM N-Phenyl-1-Naphthylamine (1-NPN; CAS 90-30-2) and the fluorescence screening system utilized three concentrations of each ligand (16 µM, 8 µM and 4 µM) and 4 µM of r-OBP1.