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Selectivity towards NH4+ and possible interferences from K+ were more accurately evaluated using the dual-excitation/dual-emission ratio, compared to a single-excitation/single-emission fluorescence scheme.
One possible method is the use of fluorogenic substrate probes to detect endogenous β-lactamase enzyme, which is naturally expressed by Mycobacterium tuberculosis, in a reporter enzyme fluorescence scheme [ 27].
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It utilizes a large-area fluorescence imaging scheme and rigorous image quantitation algorithms to enable informative analysis of cell samples attached to solid substrates.
The bond between LPS and LPS-binding peptides led to the release of dye from GO and resulted in the fluorescence recovery (Scheme 12).
Finally, we carefully analyzed pHIFU-mediated extravasation and tissue penetration of FCNPs with the blood vessel recovery in mice femoral vein using non-invasive real time near-infrared fluorescence imaging (Scheme 1b).
This design allows the implementation of both absorption and fluorescence measurement schemes and can be potentially integrated into a lab-on-a-chip system.
We have noticed a potential for non-linear (low) response in the fluorescence peak integration scheme, and thus the reported concentrations when the Hg loading (per cycle) is less than about 10 15 pg, e.g. at around 1 pg loading, the sensitivity is 25% lower than at 10 pg.
A laser-based SL instrument, recently developed in this laboratory, was used to resolve the spectra of a mixture of the dyes used in the fluorescence-based sequencing scheme.
For both probing schemes, fluorescence images are collected every 2 min using automated time lapse imaging controlled via Metamorph.
Methods details; structural overlays; KM determinations; unbiased electron density maps for 16 ligands; enzyme-NADPH and enzyme inhibitor interaction schemes; fluorescence binding assays for NADPH; growth inhibition curve for S. aureus; data and refinement statistics; chemical and physicochemical properties and kinetic data for the small-molecule inhibitors.
(b) Scheme for fluorescence analysis of peptide-decorated Au-coated VSNEA.
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