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Noteworthy, the tested fluorescent nanocomposites demonstrated high stability since no fluorescence release could be observed under the same experimental conditions.
Fluorescence release experiments.
We then analysed the time course of fluorescence release from cells transfected with equimolar mixtures of either wild-type or late domain defective variants of pCHIV/pCHIVeYFP, respectively.
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The fluorescence released following proteolytic cleavage of the substrate was determined using a fluorescent plate reader.
A color camera captured the fluorescence released from Golden Delicious apple, and the wavelength was 680 ± 10 nm.
High-resolution melting analysis of polymerase chain reaction products for mutation scanning, which began in the early 2000s, is based on monitoring of the fluorescence released during the melting of double-stranded DNA labeled with specifically developed saturation dye, such as LC-Green.
Fluorescence released by normal HDLs plus normal LDLs is set as '1.0'1.0
Cells in the presence of the dye were lysed and the extent of fluorescence released from cells was measured with a fluorescence spectrophotometer as described previously [ 23].
Furthermore, the activity of TACE, as quantitated by measuring the TACE-dependent fluorescence released by quenched QXLTM520/5-FAM QXLTM520/5-FAM (Sensolyte, Anaspec Inc., CA), decreased (~50%) in cells treated with 20 μΜ oFRETbelin.
Nanosensors of the activity of the protease granzyme B, which release a cleaved fluorescence reporter that filters into urine, enable the early diagnosis of acute transplant rejection in mice.
When entrapped in vesicles at high concentration calcein self-quenches, such that the fluorescence increases when calcein is released from the vesicles.
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