Control wells containing PCR master mix and primers without sample cDNA emitted no fluorescence after 40 cycles.
Fluorescence anisotropy was measured on a T-format fluorescence spectrometer (Quanta Master QM-1, Photon Technology International, USA).
The time-resolved, fast transient fluorescence (TRF) technique which uses a strobe master system (SMS), was employed for studying swelling and drying of disc-shaped polystyrene gels.
The final volume of the PCR reaction was 5 μL and consisted of 20 ng genomic DNA, 2.5 Fluorescence Labeling (FL) TaqMan Master Mix, and 2.5 FL 20x Assay.
PCR reactions were performed using a SYBR PCR master mix kit (AB Biosystems, Inc. Carlsbad, CA), and a Chromo4 Fluorescence Detector (Bio-Rad).
Fluorescence detection chemistry utilized SYBR Green dye master mix (Roche).
Before any fluorescein was installed, a baseline scan, measuring each eye's intrinsic fluorescence, was performed using the Fluorotron Master.
Fluorescence spectra were obtained on a Quanta Master 40 spectrofluorometer (Photon Technology International) equipped with a Quantum Northwest TLC-50 temperature controller at 25.0 ± 0.05 °C.
Fluorescence real time PCR was performed with SYBR Green PCR master mix (Applied Biosystems) following the manufacturer's protocol.
Fluorescence spectra were obtained on a Photon Technology International (PTI) Quanta Master 40 spectrofluorimeter equipped with a Quantum Northwest TLC-50 temperature controller.
At last, real-time fluorescence quantitative PCR amplification was done following the SYBGREEN PCR Master Mix Kit (ABI, Life Technologies , Inc.
