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fluorescence illuminator.
Sections were mounted with DAPI (Vectashield; Vector Labs), and images were captured using a Nikon ECLIPSE TE2000-S microscope equipped with an EXFO X-Cite 120 fluorescence illuminator.
Bright field and fluorescence images (GFP cube = Chroma, #41017; X-Cite120 fluorescence illuminator, EXFO Photonic Solutions) were acquired and image analysis performed using AxioVision Rel.
The slides were screened under the Olympus fluorescence microscope (BX 51) fitted with vertical fluorescence illuminator U-LH100HG UV, excitation and barrier filters and images were captured with a charge-coupled device (CCD) camera.
Positive signal was excited using an EXFO X-Cite fluorescence illuminator with 43HE: Cy3 filter (excitation 550/emission 605) at a set exposure time of 750 ms for all tissue.
After 10 days of culture, the cell attachment and proliferation on biomimetic MSC-Scaffold preprototypes were analyzed by fluorescence microscopy (Olympus CKX41 with CKX-RFA fluorescence illuminator, Olympus, Germany); after staining in acridine orange (AO) solution, a typical 1 10 μM AO staining concentration was used.
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Cells were screened on an E800 Nikon (New York City, NY) microscope with an EXFO (Vanier, Canada) X-cite fluorescent 120W metal halide illuminator and imaged with a DMX 1200F Nikon fluorescence sensitive digital camera.
Reaction tubes were then visualised under a conventional UV illuminator or by fluorescence microscopy.
Zeiss AxioObserver Z1 microscope (Carl Zeiss Microscopy, Thornwood, NY) was equipped with Zeiss Plan-Apochromat 150x NA1.35 glycerine-immersion objective, P-737 piezoelectric stage (Physik Instrumente, Auburn, MA), Zeiss Colibri and Lumencor Spectra-6 (Lumencor, Beaverton, OR) illuminators, and custom fluorescence cubes (Table 3).
When the precipitate is observed on an illuminator (365 nm), the fluorescence of the dye from the probe taken up by the precipitate is visualized.
After the injection of the baculovirus vector, the treated pupae were evaluated for fluorescence every day using the ATTO illuminator VISIRAYS-B.
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CEO of Professional Science Editing for Scientists @ prosciediting.com