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Stained cells with fluorescence greater than the upper limit were considered permeabilized cells.
Under saturating xylose concentration, flow cytometry analysis showed that all cells resulted in homogeneous populations, and the population with XBP showed a fluorescence greater than that without XBP.
Results are expressed as % of all cells with TMRM fluorescence greater than the threshold set by CCCP.
Analysis was performed on 200,000 cells per sample and positive expression was defined as the level of fluorescence greater than 90% of the corresponding isotype control IgG1k (BD Biosciences) or the corresponding unstained cell samples.
In an experimental variant, the cells with fluorescence greater than the threshold were counted as β-catenin-positive cells.
Cell populations that showed fluorescence greater than the brightest 5%% of uninfected cells were judged as positively stained.
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Cells with YFP-fluorescence greater than 103 were gated and denoted as TGR5-YFP-transfected cells.
pcDNA-GW/EmGFP-miR encodes a red-shifted variant of wt green fluorescent protein (GFP) that has been optimized for brighter fluorescence and greater expression in mammalian cells.
The value of Ct was determined by the first cycle number at which fluorescence was greater than the set threshold value.
The percentage of aggregates with fluorescence intensity greater than intensity indicated on the X-axis is shown on the Y-axis.
Strikingly, we find that GFP fluorescence is greater in cells treated with MMS than in those treated with NFZ (Figs. 8 and S2).
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