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In an isothermal cure, the fluorescence frequency increased linearly with the cure time until the gelation occurred.
The rate constant, KT, for the polarity change during an isothermal cure of the epoxy resin, defined as the rate constant for the linear increase in fluorescence frequency, was determined.
The total change in fluorescence frequency that occurred from the beginning of the isothermal cure to the gelation time was 1000 cm−1 and was independent of the cure temperature, implying that the chemical structure of the infinite network at the gelation time was independent of the cure temperature.
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The fluorescence (FL1) frequency distribution histogram of the haemocyte populations was obtained with the initial condition data analysed for two parameters: the complexity (SSC) and the cell size (FSC).
In animals, these fibres have been employed in catheters to provide co-localised information on microstructure and molecular function in coronary arteries, using near-infrared fluorescence and frequency domain imaging [ 29], or to visualise bile duct anatomy during surgery [ 30].
In wild type cultures, this stimulation elicited instantly sinusoidal optical signals of Vf2.1.Cl fluorescence at the frequency ν of the carrier wave (reference frequency) in virtually all cells of the network within the field of view (Additional file 1: Movie S1).
Fluorescence signal sampling frequency was 6.67 kHz; when averaged, signal traces represented an average of 2 7 sweeps, and were filtered offline at 300 1000 Hz.
The mean baseline fluorescence, amplitude, and frequency of spontaneous calcium transients in neurons were compared at different time points during imaging (Fig. 6).
With frequency-domain systems, the excitation laser is sinusoidally modulated at high frequencies, which results in oscillatory fluorescence at the same frequency as the incident light, yet the modulated fluorescence light is phase shifted and amplitude attenuated relative to the excitation source.
Telomeres were determined by Q-FISH and the distributions of telomere length are presented by fluorescence intensity versus frequencies.
Figure 2(b) shows the dependence of the fluorescence intensities at frequencies of 0 and ω on the total excitation intensity.
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