Sentence examples for fluorescence frame from inspiring English sources

Two-photon signals were weak; a single two-photon excited fluorescence frame had 0.0002 – 0.0007 photons per pixel.

A representative DIC frame and the corresponding fluorescence frame were selected and further processed with the ImageJ software.

In the living macaque eye, we observed that fluorescence emission increased in response to photoreceptor bleaching, as measured by the mean gray level of each two-photon fluorescence frame (Fig. 5 ).

DIC frames were taken with 0.1 s and fluorescence frames with 1.0 s exposure time.

Instead, up to 16,500 individual fluorescence frames (12 minute acquisition) were registered and added together to produce the final image.

When accidentally viewed from over 0.5 m, the diverging illumination hazard is well within class 1. Electronic synchronization between the camera and illumination sources provides nearly simultaneous display of white light and fluorescence frames.

Normalized, background-subtracted ΔF/F traces were calculated from these according to the equation, (3) (Δ F F ) i = F i F b a s e l i n e − 1 where F i is cell fluorescence on frame- i and F baseline is the median fluorescence intensity of the cell on that trial.

Background fluorescence was measured frame-by-frame and was subtracted from measurements of fluorescence intensity.

The left eye fundus picture shows normal disc with hard exudates superonasal and inferotemporal to macula Fig. 2 FFA in the right eye showed presence of three hypofluorescent lesions in posterior pole in early frames with progressively increasing hyper fluorescence in late frames due to leakage.

A CCD camera (Rolera-XR Fast 1394, QImaging) mounted to a microscope with a 40×/0.8 NA water-immersion objective (Leica Microsystems) was used to collect fluorescence at a frame rate of 7 Hz.

The mask is then used to find total mCherry and YFP fluorescence in the frame.