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Fluorescence FISH with rRNA-targeted probes is a staining technique that allows phylogenetic identification of bacteria in mixed assemblages without prior cultivation by means of epifluorescence and confocal laser scanning microscopy, or by flow cytometry.
Her-2/ neu gene amplification is primarily detected by in situ hybridization and uses fluorescence (FISH) to detect the signals.
It is analyzed by fluorescence (FISH) or chromogenic (CISH) in situ hybridization to measure the gene copy number.
Tissue based assessment of HER2 protein expression levels is commonly achieved using immunohistochemistry (IHC), whereas tissue based analysis of HER2 amplification is mostly done by in situ hybridization (ISH) techniques either fluorescence (FISH) [ 4] or chromogenic (CISH or SISH) [ 5].
Standard testing methods include analysis of HER2 protein expression on the cell membrane by IHC and/or evaluation of HER2 gene copy number by in situ hybridiztion (ISH), most commonly fluorescence (FISH), but also silver (SISH) or chromogenic (CISH), using DNA-based probes targeting the HER2 gene locus and chromosome 17 centromere (CEP) [ 1].
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In situ fluorescence hybridization (FISH) analyses were carried out primarily as described by Taneja et al, 1995 [15] and Dansithong et al, 2005 [46].
Representative images were captured and stored using the Isis colour fluorescence and FISH imaging system (Metasystems, Germany).
All 7 cases that exhibited Her2 3+ staining were found to be HER2-amplified on silver ISH and fluorescence ISH (FISH) with 100% concordance between the two methods.
Others have suggested that fluorescence in fish increases with depth, and thus serves a visual, not photoprotective role (Michiels et al., 2008; Meadows et al., 2014).
In vitro studies have revealed a difference in average telomere length after X-irradiation using quantitative analysis of emitted fluorescence (Q-FISH) [35], [36].
One reason why red fluorescence in fishes may have escaped attention is because fluorescence is usually observed during night dives using strong UV light sources.
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