Epifluorescent images were visualized on a Leica microscope (47-nm cyan fluorescent protein, fluorescence filter set 47 (EM BP 436/20, BS FT 455 and EM BP 480/40); Leica Microsystems, North Ryde, 2113 Australia).
Depending upon the assay, one of the following three fluorescent filters was used: FITC HYQ fluorescence filter (460-500 nm); TX RED HYQ fluorescent filter (532-587 nm); and ultraviolet filter (325-375 nm).
The fluorescent transformed seeds were selected under an inverse microscope equipped with a DsRed fluorescence filter (Axiovert 200M; Zeiss AG, Germany) and put on soil to grow the next generation.
Cells were visualized with a microscope equipped with fluorescence filter.
The images were viewed under the fluorescence microscope (×1,000 amplification) with a green fluorescence filter.
The combination of an LED and a fluorescence filter provides the opportunity for fluorescence quantification.
Microdissection was conducted by a DPSS laser beam at 349 nm wavelength, aperture of 12, speed of 10, power of 50 60 μJ and pulse frequency of 2895 Hz under a Leica LMD-BGR fluorescence filter system at 10x magnification.
Ten microliters of the suspensions were placed on slide glass and used for microscopic observation by fluorescence microscope (Nikon, Japan) with a green fluorescence filter (Green Excitation 460 500 nm, Emission 510 560 mm).
To further confirm the apoptotic changes in P815 and BSR cells, Anexin V biotin-streptavidin FITcellsined cells were visualized with a microscope equipped with fluorescence filter (OLYMPUS OM52).
Finally, sections were cover-slipped and scanned with the NanoZoomer 2.0-HT System (Olympus) at 20× resolution using TRITC fluorescence filter, and images were acquired using NDP scan 2.5 software (Olympus).
Fluorescence expression patterns were observed with a Nikon SMZ800 dissection microscope equipped with a fluorescence filter.
