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The dominant fluorescence component with τ 0.93 ns clearly corresponds to that measured for multiphoton induced hyperluminescence from solutions of 5-OHTrp (τ 0.91 ns [29]) and confirms the green emission as arising from 5-OHTrp.
With KCNE1, the channels remained closed during the fast fluorescence increase and, after this initial delay, the channels opened with a single timecourse, similar to the slow fluorescence component.
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Three fluorescence components appear at zero glucose concentration and systematically transition to three components at high glucose concentration.
This study evaluated the Excitation Emission Matrix (EEM) fluorescence method combined with Parallel Factorial Analysis (PARAFAC) modeling to resolve DOM fluorescence components, and their distribution from near-shore to off-shore on the southwest (SW) Florida Shelf.
The methods employed were able to: (1) resolve sources of the individual DOM fluorescence components of the bulk DOM; (2) generate spatial and temporal distribution maps of each of the components on the shelf; and (3) provide insights into biological, chemical, and physical processes that control the DOM variability of each of the resolved DOM components.
Therefore, in order to encompass these spectral features and gain further insight into the cosmic ray composition variation along the transition region, enhancements to the surface and fluorescence components of the baseline design are being implemented that will lower the full efficiency regime of the Observatory down to ∼1017 eV.
PARAFAC provides a way to decompose a dataset of EEM into individual fluorescence components [39, 40].
Two different channels were selected to obtain high-contrast images of collagen and fluorescence components, respectively.
The fluorescence components can be correlated with the size, construction, and other physical aspects of cells.
We next used the combination of intensity and lifetime quenching ratios to calculate the relative amount of static quenching, dynamic quenching, and unquenched fluorescence components.
In the case of lifetime multiplexed studies, unmixing algorithms applied to the decaying portion of the TPSFs have been employed to recover the fractional contribution of the different fluorescence components and perform FMT based on unmixed time-independent signals [ 10].
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