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Hence, the evaluation of photo-stability of a fluorescent tracer in combination with the fluorescence camera intended for in vivo use is a key aspect in the translational process (van der Wal et al., 2016).
Nevertheless, the more sensitive IVIS200 fluorescence camera did demonstrate a faint fluorescent signal in both nodes ex vivo.
Since IR700 is also a fluorescent dye, we were also able to observe PIT effects with a fluorescence camera.
Quantitative ex vivo images of the excised tissue specimens were acquired with the portable gamma camera and with a more sensitive preclinical fluorescence camera system (IVIS200, Xenogen, Caliper Life Sciences, Hopkinton, MA) to determine the radioactive and fluorescent signal intensities as described previously.
(D) Tissue sections were visualized and photographed using an AxioCamMRm3-2 fluorescameracattachedtoched to a Zeiss Imager M2 fluorescence microscope at 400× magnification and software enlarged.
Stained tissue sections were photographed using an AxioCamMRm3-2 fluorescameracattachedtoched to a Zeiss Imager M2 fluorescence microscope at 400× magnification.
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There are multiple near-infrared fluorescence cameras available.
This work indicates that adding FLTI to conventional intraoperative fluorescence cameras might extend the value of such devices for detecting small tumors during surgeries in which pooled hemorrhagic fluid/ascites are likely to occur.
A Germanium filter transmitted the infrared light to the thermography camera and reflected the fluorescence to the fluorescent camera.
Images were recorded by a high-resolution fluorescence CCD camera (CoolSNAP™ HQ2, Photometrics®) with NIS-Elements Advanced Research (Nikon) software.
Monolayers were viewed under identical fluorescence and camera exposure to allow comparison between conditions.
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