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Compared to other fluorescence analytical methods, the fluorescence sensor we design is neither the traditional fluorescent "turn on" probes nor "turn off" probes.
Three-dimensional fluorescence spectroscopy, a kind of new fluorescence analytical technologies developed in the past 20 years, can visually show the microenvironment and conformational changes under different conditions of Trp in protein molecules.
A 3D microfluidic paper-based fluorescence analytical device with hollow channels based on the turn-on switching of a resonance energy transfer triggered by the OH induced cleavage of a DNA strand was successfully constructed.
It is a new fluorescence analytical method that target object indirectly control the surface passivation degree of CQDs so that it can realize the detection of the target object.
Taking advantage of the effective fluorescence quenching effect of K+, a highly sensitive CD-based fluorescence analytical system for label-free detection of K+ with limit of detection (LOD) 1.0 × 10−12 M was established.
To confirm that the RTX domains were associating and that this assembly was correlated with the changes in ThT fluorescence, analytical gel filtration and UV absorbance were used to assess changes in the concentrations of soluble RTX protein during aggregation experiments.
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Additionally, the use of the HPLC-fluorescence analytical method, although improving the speed of analysis, may have missed low levels of PAH contamination of public health relevance for vulnerable populations.
Spectral (UV Vis, FT IR, NMR and fluorescence) and analytical data allowed the structures of the synthesized compounds to be established.
Polarizing Light Microscopy (PLM), X-Ray Diffraction (XRD) and X-Ray Fluorescence (XRF) analytical techniques are used to characterize both the raw materials collected in Veneto and Piedmont (Italy) and the black earths available on Italian and German markets.
Characteristics tested were fluorescence intensity, analytical sensitivity, kinetics of complex formation and signal stability.
In previous reports, the fluorescence anisotropy, analytical ultracentrifugation, and NMR assays confirmed a direct interaction between Bcl-xL and BAK1 [ 5, 20].
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