Stained slides were examined using Olympus BX51 fluoresces microscope.
Thus, in the presence of PI, cells with permeable membrane (lysed or damaged cell membrane) will fluoresce under the fluorescence microscope.
Tetracycline enters bone attached to calcium and because it fluoresces under a fluorescent microscope, is used as a means of quantifying certain dynamic parameters of bone turnover.
The H2B-GFP fusion protein decorates chromosomes and allows observation of live cells that fluoresce green under the fluorescence microscope [ 18].
The green and red fluorescing cells observed under fluorescence microscope were counted from at least five different spots and their mean is expressed as % apoptotic cells.
One common method uses proteins that fluoresce when viewed under a fluorescence microscope each time the protein senses increases of calcium.
To assess the degree of defense response around the primary infected cell, the diameter of brightly fluorescing area was measured under a fluorescence microscope with a micrometer.
The apoptotic cells were fluoresced bright green, and were detected by the fluorescence microscope with NIBA filter.
TUNEL+ apoptotic cells, which fluoresce bright green, were viewed with a Nikon Eclipse E1000 fluorescent microscope (Nikon Corp., Tokyo, Japan) equipped with a FITC filter.
