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About 30 ml of buffered rumen fluid was added into syringes containing the samples.
For every 1 mL of sample taken, 1 mL of simulated intestinal fluid was added to the reaction mixture.
For every 1 mL of sample taken, 1 mL of simulated gastric fluid was added to the reaction mixture.
Rumen fluid was added to the buffered mineral solution with constant stirring, while maintained in a water bath at 39 °C.
The extraction fluid was added to 100 g of crushed soil sample maintaining 20 1 (L/S) ratio, and then rotated at 30 ± 2 rpm for 18 ± 2 h.
The cells were harvested and taken in scintillation vials, scintillation fluid was added and counts were taken.
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Here another fluid is added that has a still greater affinity for the metal values, picking them out of the extraction solution.
No dispersant fluid is added during the nanofluid preparation.
The results generalize existing theories by accounting for mass flow through the vessel floor and changing power supply, and delineate a term which describes the influence of vapour escape through the interface; the latter term quantifies the ultimate disappearance of the liquid when no fluid is added to the system.
In order to define the role of soluble HS/heparin on the binding of VEGF-A and its surface receptors, VEGFRs were crosslinked to the bottom of wells and soluble HS GAGs or porcine ocular fluid were added with a fixed amount of VEGF-A.
Sodium acetate (20 mM final conc)., sodium formate (60 mM final conc)., coenzyme M (10 µM final conc)., and vitamin-supplemented clarified rumen fluid were added to sterile media, before inoculation with 0.5 ml of an actively growing culture of M. ruminantium, then gassed with H2 plus CO2 (4∶1) to 180 kPa overpressure.
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CEO of Professional Science Editing for Scientists @ prosciediting.com