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Synovial fluid samples were treated with hyaluronidase.
Some blister fluid samples were treated with APMA.
Synovial fluid samples were treated with hyaluronidase before the cell count.
Before analysis, synovial fluid samples were treated with hyaluronidase (Hylase Dessau; Riemser Arzneimittel, Greifswald, Germany) at 37°C for 30 minutes and centrifuged at 3,500 rpm for 10 minutes.
Due to viscosity, all synovial fluid samples were treated with 5 U/mL of the hyaluronan-specific Streptomyces hyaluronidase (Sigma-Aldrich, St . Louis MO, USA) overnight at 37°C prior to further analysis.
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To extract and concentrate calcium-containing crystals, Swan et al. used a protocol where the synovial fluid and articular cartilage samples were treated with papain and sodium hypochlorite.
In group two the specimens and material samples were treated with PACT-Fluid Endo for 1 minute and the PACT Light source for 1 minute.
The samples were treated with HPPIB.
Bioreactor and foamate samples were treated equally.
All samples were treated for 1 min.
All samples were treated in duplicate.
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