Sentence examples for fluid samples were then from inspiring English sources
The rumen fluid samples were then centrifuged at 4000 rpm for 5 min and the supernatant obtained was used further for the DNA extraction.
The BAL fluid samples were then centrifuged for 7 min at 500 ×g, and the BAL cells were collected and resuspended in 1 mL of 0.9% NaCl solution for evaluation.
Vaginal fluid samples were then subjected to eight separate taxon-directed 16S rRNA gene quantitative PCR assays for the detection and quantification of individual bacteria which have been described elsewhere [ 11].
Scintillation fluid (10 ml) was added and the samples were then assayed for [H] glucose content in a liquid scintillation spectrophotometer.
The excess fluid was removed using absorbent paper, and the samples were then visualized using a transmission electron microscope.
Samples were then solubilized in Ultima Gold scintillation fluid and analyzed by liquid scintillation counting.
The samples were then processed to produce two fractions: strained ruminal fluid (SRF) and liquid-attached bacteria (LAB).
Samples were then processed and analyzed for LPS and VFA using the same procedure described earlier for rumen fluid samples.
