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100 pleural fluid samples were processed by NALC-NaOH method [12].
Blood and follicular fluid samples were processed within two hours of collection.
BAL fluid samples were processed as described previously (Krug et al. 2001).
Follicular fluid samples were processed according to the manufacturer's instructions using a MARS-Hu-14 immunodepletion column from Agilent Technologies (Santa Clara, CA).
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After incubation for 3.0 h, the extracellular fluid was collected and the samples were processed to extract ADO as reported previously [28], [76]; briefly, the extracellular fluids were rapidly collected into microcentrifuge tubes and centrifuged at 14,000 x g for 1.0 min. Supernatants (400 µl) were placed into separate tubes and deproteinated with 20 µl of 100% trichloroacetic acid.
Additional file 3: Correlation of age with cathepsin S (CTSS) and B (CTSB) activity on serum and synovial fluid (SF): serum and SF samples were processed and run as indicated in the Materials and methods section.
All samples were processed individually.
Samples were processed within 1 h.
Samples were processed and analyzed by Cumberland Valley Analytical Services , Inc
Samples were processed.
Samples were processed anonymous.
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