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The synovial fluid samples were eluted from the filter paper by immersion in 250 μL of stirred ice-cold PBS for 10 min.
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Samples were eluted from the beads by boiling at 95 °C for 5 min.
The peptide samples were eluted from the beads using 0.15% TFA.
Samples were eluted from the stage-tips in 75% ACN + 0.1% TFA and lyophilized.
The PMMA-graft-PDMS samples were eluted from the components of higher PMMS contents to those of lower PMMA contents, according to reversed-phase adsorption.
Purified MB or S-MB samples were eluted from reverse-phase HPLC as indicated above.
For Western blot analysis, samples were eluted from beads with MURB sample buffer.
RNA samples were eluted from the columns with 40 μl of RNase-free water.
Samples were eluted from magnetic beads in 40 μl of EB Buffer.
The sample was eluted from the column with hexane and concentrated into small volume (0.5 ml) of hexane.
The sample was eluted from the cartridge with 4 ml acetonitrile (Sigma).
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