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Extracellular fluid samples were analysed in a biochemical bedside analyser.
Lavage fluid samples were analysed for protein concentration using BioRad assay reagent (BioRad Laboratories, Hemel Hempstead, UK) and for cytokines including interleukin-6 (IL-6), macrophage inflammatory protein-2 (MIP-2), keratinocyte-derived chemokine (KC) and interleukin-10 (IL-10) using ELISA kits (R&D Systems, Abingdon, UK).
Records of 4,955 cerebrospinal fluid samples were analysed.
Differences in the matched blood and synovial fluid samples were analysed by the paired t test.
The survivin levels in the matched blood and synovial fluid samples were analysed by the paired Student t test.
The correlations between the levels of various markers in the pleural fluid samples were analysed using the Pearson correlation analysis.
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Ileostomy fluid homogenate samples were analysed for iso, as well as glucose and fructose as hydrolysis products using GC (Agilent 6890) and a capillary fused silica column CP-Sil 8CB (15 m × 150 μm × 0·15 μm) with FID.
For quantification, triplicate samples were analysed.
The samples were analysed using the LSRFortessa flow cytometer.
Samples were analysed under an epifluorescence-equipped light microscope (Olympus).
Samples were analysed with FACSVerse (Becton Dickinson) and FlowJo software.
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