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The majority of CellSearch® images analyzed were from patients with M0 breast cancer, with <5 CTCs in their blood sample and from patients for whom blood sampling for CTC detection was performed before the administration of systemic treatment (Table 1).
In a study comparing two commercially available assays (CellSearch® and AdnaTest®) in the same metastatic BC patient samples, the concordance between the two assays was 64% for CTC detection and 50% for HER2- positive CTC detection [ 42].
This model allowed taking into account the major challenges for CTC detection and enumeration: (1) the discrete process; (2) the Poisson-distributed sampling error; (3) the overdispersion; and (4) the high number of CTC counts equal to 0. This report represents the first use of a cell lifespan model to describe CTC kinetics.
blood samples per patient, method of CTC isolation and enrichment, molecular technique, target gene, and/or antigen used for CTC detection, in vitro sensitivity of each molecular method (if assessed), and no.
Despite the numerous methods for CTC detection a major question that arises is whether these assays give the same information when analyzing the same clinical samples.
The correlation remained significant when the cut-offs for CTC detection with the CellSearch were set at ⩾1 and ⩾2 CTCs (Table 2).
Stott and colleagues [ 34] recently reported improved sensitivity of the CTC-chip for CTC detection in patients with localized prostate cancer.
In summary, with lower and inconsistent SEN estimates for CTCs detection in GC, CTCs detection alone cannot be recommended as a screening test of GC.
From each patient we obtained peripheral blood for CTCs detection and corresponding paraffin-embedded tumor tissue.
The QRT-PCR used in our study is not the only available assay for CTCs' detection.
Blood samples were subjected to CTC detection assay, and then detectable cells were counted by fluorescence microscopy.
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