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In this review, we first discuss tools to capture changes in the fluid milieu of organoid cultures both in the organoid exterior as well as the luminal side of the organoids.
As verified through a proteomic approach, the NB/NLP formed under these conditions were seen to adsorb or "hijack" calcium and apatite binding proteins from the fluid milieu used [2].
In this study we assayed FF from anonymous oocyte donors undergoing IVF oocyte retrieval, with the hypothesis that the proteins identified will allow for a better understanding the follicular fluid milieu in normal healthy reproductive age women.
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Gastrointestinal fluid is a complex milieu and it is recognised that gut drug solubility is different to that observed in simple aqueous buffers.
Although multiplex bead assays do not provide information regarding the cellular source(s) of cytokines, the results nonetheless underscore the broad effects of ascites fluid on the cytokine milieu in EOC.
The first collected fluid, reflecting tracheo-bronchial milieu, was sent for microbiological culture.
This group of proteins was explored by sorting from the over-expressed genes, the proteins secreted in the external milieu (uterine fluid) where calcification takes place.
The solubility of oxygen in interstitial fluid (in the tissue milieu) is not known, and it is uncertain whether it differs from that of oxygen in water or serum.
While uterine fluid represents the preimplantation milieu of the embryo, the nutritional contents of this fluid have not been fully characterized in the human and it remains unclear how these may be altered by factors such as female age, lifestyle and disease.
This will allow the clinician to take full advantage of the many potentialities of salivary fluid as an ideal milieu for the diagnosis of pSS and other systemic diseases and for the identification of the pathogenetic processes underlying glandular and systemic disorders.
Analysis of the lavaged lung fluids suggested that the milieu of the inflamed lung amplified metal-mediated oxidant chemistry to jeopardize the compromised cardiopulmonary system.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com