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After centrifugation at 6500 rpm for 5 min and the supernatant fluid being discarded, the resulting precipitate containing MPA-capped ZnCdSeS QDs was dissolved in distilled (DI) water and some tetramethylammonium hydroxide (TMAOH) was added to make it clear.
The supernatant fluid was discarded.
Remaining IV fluid was discarded.
To obtain a headspace of ~0.3 l, Ar/CO2 was pumped into the settler and the excess fluid was discarded.
The remaining supernatant fluid was discarded and the protein pellet resuspended in 200 μl of 1 N NaOH.
The supernatant fluid was discarded, and the pellet was resuspended in a volume of cold water equal to that of the original volume of the suspension.
The collected fluid was discarded and 500 μL extraction solution (Illustra™, GE Healthcare, Pittsburg, PA, USA) was added again to the column.
The supernatant fluid was discarded, the biliverdin pellet was frozen at −80°C and then freeze-dried using a FreeZone Plus Freeze Dry System (Labconco, Kansas City, MO USA).
The authors concluded that the association of AKI with inhospital mortality was still significant even when the appropriate fluid challenge requirement is discarded.
The buffer sharing is unrestricted as long as there is available space; if the buffer is full, the necessary amount of fluid from the most demanding flows is discarded.
Ten millilitre samples of PD fluid from each drained bag will also be obtained before the dialysis effluent is discarded.
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