Key aspects considered include: i) donor animal species and number of animal used, ii) diet fed to donor animals, iii) collection and processing of rumen fluid as inoculum, iv) choice of substrate and incubation buffer, v) incubation procedures and CH4 measurements, vi) headspace gas composition and vii) comparability of in vitro and in vivo measurements.
In conclusion, the two tested modifications increased the protozoa numbers in continuous-culture fermenters, and the FIL allowed maintaining a proportion of holotrich protozoa similar to that in the ruminal fluid used as inoculum.
Use of preserved rumen fluid (RF) as microbial inoculum for in vitro gas production techniques (IVGPT) would be of practical importance when access to ruminally cannulated animals is limited.
The ruminal fluid was used as the inoculum for batch culture.
A100-μL aliquot of the supernatant fluid was used as the inoculum.
A culture of the marine green alga Nanochloropsis salina was used as a biocatholyte, and a rumen fluid microbiota was the anodic chamber inoculum.
The model and SOP were shown to be rugged with respect to slight changes in fluid mixing intensity, water chemistry (saturation index), inoculum size, and organic loading.
Leaf samples were incubated with each rumen fluid (i.e., RC and RX inoculums) in 3 runs on different weeks.
To reflect human sepsis as closely as possible, we used a CLP model, which has advantages over bacterial inoculum and lipopolysaccharide models [45], incorporating fluid and antibiotic therapy to recreate haemodynamic and metabolic phases of treated human polymicrobial sepsis.
A sham inoculum was prepared by diluting sterile allantoic fluid 1 100 in BHI.
