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For adaptation to the embryonated chicken egg, MV was passaged thrice in the allantoic cavity, and the allantoic fluid harvest was stored at −20°C to create a virus pool.
Using a 25-mm circle card (ANTEC, UK), 100 μl of the allantoic fluid harvest was mixed with 100 μl of standard washed Macaca mulatta red blood cells (mRBCs), and the card was rocked for about 8 minutes.
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Pre; synovial fluid harvested at surgery.
Acute; synovial fluid harvested at 3 to 4 days after surgery.
A NASBA/ECL technique has been developed allowing the detection of RNA from avian influenza virus subtype H7 derived from allantoic fluid harvested from inoculated chick embryos and from cell cultures.
The end-target in RA being the joint, we set out next to analyze the CD4+NKG2D+ expression levels within mononuclear cells in the articular fluid harvested after Ficoll density gradient centrifugation from one RA elbow and 5 controls (three knee osteoarthritis and two non auto-immune knee arthritis) patients.
Allantoic fluid harvested from inoculated eggs showing embryo death tested positive for hemagglutinating agents.
HAI test antigens were allantoic fluid harvests from infected embryonated hen's eggs (whole-virus antigens).
The level of vascular endothelial growth factor in vitreous fluid harvested at operation was determined.
However, V γ9V δ2 T cell did not respond to the ascites fluid harvested after i.v. zoledronate injection.
Besides, fluid harvested from axillary drainage stimulated HER2-positive mammary carcinoma cell growth in a two-dimensional growth assay.
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