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Corrections for the flow rate fluctuations were performed using toluene as an internal standard.
Corrections for flow rate fluctuations were performed using toluene as the internal standard.
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Fluctuation assays were performed using these 43 strains to determine the mutation rate at the URA3 and CAN1 genes.
Simulations were performed using PNNL Institutional Computing Resources.
Simulations were performed using Matlab.
Computations were performed using the program Salvador, which calculates both m, the mean number of mutations occurring in a culture, and 95% confidence intervals from the fluctuation data (Zheng 2002, 2005, 2007).
qPCR was performed using Power SYBR Green PCR Master Mix Applied Biosystemss).
Broadband recordings (30 min duration) of extracellular voltage fluctuations were performed with 30 kHz sample rate (Cerebus, Blackrock Microsystems).
An analysis of the sources of fluctuations was performed in order to optimize future detector designs.
Fluctuation tests were performed as previously described [4], [57] with modifications.
For each strain, at least two independent fluctuation analyses were performed.
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