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In the age of fast computation techniques, higher-order velocity fluctuations were analyzed using Fourier transformation (Salazar and Collins 2012), but it was also rated as an ill performer for signature characteristics of time series in hydraulic engineering spectrum (Gurley et al. 2003).

The video image sequences of the GUV thermal fluctuations were analyzed using several custom-made software to perform contour extraction, contour cleaning and fluctuations analysis procedures described previously (Mitov et al., 1992).

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Most often, fluorescence intensity fluctuations are analyzed using temporal autocorrelation analysis (Fig. 6C, D), as done in this study, but other approaches can also be applied [45].

All the sediment samples were collected on a seasonal basis for the assessment of fluctuation in 2014 2015 and after preparation samples were analyzed using standard procedure.

The structural fluctuations of HIV-1 protease in interaction with its substrates versus inhibitors were analyzed using the anisotropic network model.

Structural dynamics were analyzed using the diffracted X-ray tracking method, showing that fluctuations were suppressed upon antigen binding.

Root mean-square deviation (RMSD) and root mean-square fluctuation (RMSF) calculations per simulation frame that measured backbone flexibility (Cα atoms and amino-acid residues) were analyzed using CPPTRAJ (http://ambermd.org/tutorials/analysis/ cpptraj 66 and visualized in VMD (http://www.ks.uiuc.edu/Research/vmd/).uiuc.edu/Research/vmd/

Images were analyzed using Gap Light Analyzer41.

Data were analyzed using one-way ANOVA.

Data were analyzed using Prism 5.0 (GraphPad Software).

Plates were analyzed using a Molecular Devices ImageXpress High-Content Analysis SysteMolecular Devices ImageXpress High-Content

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