The result showed that sensitivity of microphysiometer is significantly enhanced to sense the fluctuation of cellular metabolism rapidly and sensitively in real-time detection of living cells under physiological condition.
Notably, the fluctuation of cellular factors is generally attributed to oscillations of gene expression.
In keeping with Nox activity, finely controlled, modest fluctuations of the cellular redox status have been shown to be capable of reversible modulation of signalling cascades.
Here, dynamical noise can play a role due to the low concentrations of molecular species and random thermal fluctuations of the cellular environment.
Physico-chemical stochastic fluctuations of the cellular environment introduces small variability in the spliceosome efficiency and perhaps results in imperfect selection of splice sites that eventually produces many lowly expressed alternative transcripts, as is observed in our data [ 6, 13, 19, 33, 43, 44].
In the rat model of LID, fully denervated animals show a high sensitivity to the fluctuations of extra-cellular DA induced by l-dopa treatment (Dethy et al., 1999), often showing dyskinetic behaviours even after the first l-dopa dose (Winkler et al., 2002 and Vinuela et al., unpublished data).
An increase in the fluorescence of the cells confirmed that BODIPY-appended calix[4]arene sensed the fluctuation of the cellular pH during early cell apoptosis.
AMPK plays an important role in fatty acid oxidation, glucose uptake, and hepatic lipogenesis by reacting to the fluctuation of the cellular AMP ATP ratio (Hardie et al., 2012; Inoki et al., 2012).
One possible source of stochasticity is the effect of cellular fluctuations on the activity or stability of a protein or RNA transcript.
