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The photon counting histogram (PCH) and similar techniques, such as fluorescence intensity distribution analysis (FIDA), are fluctuation techniques designed to analyze the oligomeric status of fluorescent species and have been applied to both in vitro and in vivo systems.
Fluorescence correlation spectroscopy (FCS) and fluorescence cross-correlation spectroscopy (FCCS) [22] [26] are fluctuation techniques that analyze protein mobility, concentration, and protein-protein association (Figure 1A, 1B), and have recently been applied to live yeast cells expressing autofluorescent proteins (AFP) at the endogenous levels [27], [28].
Several fluctuation techniques were used to obtain this overall picture of paxillin dynamics, including single-point, circular and line scanning FCS, RICS, and temporal correlation using fast cameras.
Various fluorescence fluctuation techniques have been extensively applied in chemistry, biophysics, biology and related fields, in studies of photochemistry, diffusion properties, chemical kinetics, aggregation, conformational fluctuations [ 3– 7], both in vitro [ 8] and in living cells [ 9– 11], on small molecular ensembles as well as on single molecules [ 12, 13].
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Potential recharge characteristics of the water harvesting structures were computed using the storage volume fluctuation technique.
The model results are representative of rainfall and water level measurements in the catchment, and compare well with water table fluctuation technique, although estimates are dissimilar to previous estimates within the catchment.
Like NMR, it is an equilibrium fluctuation technique.
For this purpose, a variety of fluorescence fluctuation microscopy techniques can be applied.
Our method is based on spatio-temporal fluctuation correlation techniques in which several points in space are cross-correlated and the precise time required for the same molecule to be found in a given location away from the position at time zero can be measured.
These fluctuations are used in fluctuation-sensitive techniques to gain better insight into the underlying physical evolution of the system, and are therefore of great interest [ 2].
Among the different recharge estimation methods, water table fluctuation (WTF) technique is the widely applied method for quantifying recharge rates (Healy and Cook 2002).
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