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Genes in CS8 were constitutively expressed in six tissues, and part of them showed fluctuating expressions in SAM38D.
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Many studies over the years have demonstrated that undifferentiated mESC culture contains heterogeneous populations which are identified by fluctuating expression of transcripts and cell-surface markers [1] [10].
Maintaining undifferentiated mouse embryonic stem cell (mESC) culture has been a major challenge as mESCs cultured in Leukemia Inhibitory Factor (LIF) conditions exhibit spontaneous differentiation, fluctuating expression of pluripotency genes, and genes of specialized cells.
This mechanism ensures that with fluctuating expression levels, CENP-A remains mainly centromere restricted, and may prevent potential neocentromere seeding.
Analyzing development-related miRNAs expression at 14 developmental stages based on clone-sampling and stem-loop RT PCR, we discovered an unusual abundance of 33 sequences representing 12 different miRNAs and sharply fluctuated expression of miRNAs at larva-molting stage.
The model predicts fluctuating expression levels for several factors involved in pluripotency maintenance, largely consistent with empirical observations presented here or reported previously.
We also demonstrate that ERK phosphorilation is altered and being anti-correlated with fluctuating Nanog expression – in accord with model simulations.
(E ) RNA-seq and quantitative polymerase chain reaction (qPCR) show fluctuating mean expression ± SD of the lincRNA NONCO7761 (3100003L05Rik ) over 24 hr.
Although the heat shock every 6 hours is intense from the heat shock standpoint, the short half-life of the ARF protein results in fluctuating ARF expression, rather than sustained high levels.
Taking into consideration the high frequency of fluctuating gene expression patterns we considered only transcriptional changes above or below the [+1 -1] M' thresholds as a function of incubation time during gene function analysis.
In the corpus callosum, genes related to growth, survival and functions of glial cells fluctuated their expression.
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