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We confirmed 80% knockdown of synoviolin mRNA and protein by RNA interference in RA FLS (Figure 4A).
In comparison with OA FLS or OA dermal fibroblasts, IL-17-induced synoviolin expression was more marked in RA FLS (Figure 1C).
Both IL-17RA or IL-17RC siRNA lead to near complete abrogation of IL-17-induced synoviolin expression in RA FLS (Figure 3D).
Synoviolin RNA interference increased SNP-induced apoptosis by 4 fold in RA FLS (Figure 4B, P<0.05) demonstrating the specificity of its contribution.
Stimulation with IL-1β caused a 2.5-fold increase in p-c-Jun DNA binding compared to unstimulated FLS (figure 4D).
32 In initial experiments, we found that TSA treatment had no effect on the magnitude or kinetics of inhibitor of κBα (IκBα) phosphorylation or degradation in RA FLS (figure 5A).
Similar(53)
MtPykA phosphorylation was also confirmed by GST-PknJ-FL (Figure S5).
The fluctuation pattern of plasma miR-92a detection levels in relation to clinical status in T-cell NHL was similar to that of DLBCL and FL (Figure 3C).
The transcripts defined as full-length mRNAs (FL, Figure 1) are the ones showing a complete ORF.
Full-length IP3R1 tagged at its N-terminus with EYFP (FL; Figure 1C) was localized in the ER of COS-7 cells.
In other locations, heat index and air temperature differ more during summer (e.g., Houston, TX, and Miami, FL; Figure 4).
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