Exact(10)
Comparison of traditional LP over fully fuzzified-LP (FLP) model has also been addressed to elucidate the advantages of FLP in TOC.
Transient expression of FLP in the targeted hESCs led to the excision of the selection marker from the knock-in allele.
Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermo-instability of the protein [6].
There is a striking difference, though, between Cre and Flp in how they assemble their respective active sites.
Cre is akin to topoisomerase, and contrary to Flp, in its mode of active site assembly and strand cleavage.
However, Cre resembles Flp in the organization of its recombination synapse and the conformational dynamics of strand exchange [3], [4].
Similar(49)
(A) SiRNA transfected Flp-In-293 cells were treated with tetracycline as indicated.
To this end, we generated Flp-In-293 cells to stably express wild-type or mutant Sec61β (Fig. S2F).
A CHO cell line stably expressing ROBO1 fused with an HA tag (ROBO1-HA) was generated using the Flp-In System (Life Technologies Japan Corp., Tokyo, Japan).
For flow cytometry, wild type or ROBO1-HA-expressing Flp-In-CHO cells were incubated with primary antibodies for 1 h at a concentration of 1 μg/mL.
(c) Dose-dependent reactivity of the anti-ROBO1 IgG by cell ELISA (filled circle, wild type CHO-cells; open circle, ROBO1-HA-expressing Flp-In-CHO cells).
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