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The resulting slurry was transferred to a gravity-flow column and washed twice with 50 ml of lysis buffer.
IMAC purification was performed for rUbi using a 1 mL Ni-NTA Agarose fast flow column (GE Healthcare).
The curtain flow column design allows for separations that are faster and/or more sensitive than conventional columns, depending on how exactly the curtain flow column is configured.
To gain more insight into the design of a radial flow column, the velocity profile and resin distribution in the radial flow column were calculated.
This LC-based approach is similar to continuous-flow column (i.e., breakthrough) methods.
Removal of cadmium by xanthated chitosan was investigated in a packed bed up-flow column.
Removal of hexavalent chromium by xanthated chitosan was investigated in a packed bed up-flow column.
We used an axial flow column and a miniaturized radial flow column with a ratio of 1.8 between outer and inner surface area, both with a bed height of 5 cm.
The simultaneous anaerobic transformation of tetrachloroethene (PCE) and carbon tetrachloride (CT) was evaluated in a continuous flow column.
For example, when mass spectral detection is employed, the curtain flow column can yield separations that are 5-times faster than conventional columns when the curtain flow and the conventional columns have the same internal diameter.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com