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Differential expression analysis of two flowering phases was performed using the DESeq R package (1.8.3).
Expression analysis of RNA-seq and qRT-PCR identified novel and conserved miRNA that were differentially expressed during flowering phases.
To identify differential expression of miRNAs between two flowering phases, normalized miRNA levels were compared between PS1 and PS4.
As shown in our results, three members of cen-miR167 family were all expressed at low levels during both flowering phases and targeted auxin response factor.
In this study, we employed Solexa technology to sequence four small RNA libraries from two flowering phases to identify miRNAs related to floral development.
In our study, cen-miR156 and cen-miR172 were identified during both flowering phases, suggesting that they may have roles in flowering and determining identity of floral organs in C. ensifolium.
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The abundance and flowering phase of these species differ among regions even in a small country such as Austria [ 10].
In plants, miRNAs are implicated in diverse aspects of plant growth and development, including leaf morphology and polarity, lateral root formation, hormone signaling, transition from juvenile to adult vegetative phase and vegetative to flowering phase, flowering time, floral organ identity and reproduction [ 13, 14].
They did find that heat shock was likely to increase at times when plants are known to be especially sensitive, like during the flowering phase.
Leaf area continued to increase during the flowering phase, notably in NL-6, the earliest genotype.
Figure 6 Weekly stigmatic receptivity and ovule and bract development during the female flowering phase in 2006 and 2007.
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