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We have also included experiments on a short vernalization of Col FRISf2 that demonstrate that partial vernalization of this faster flowering line does not reveal a differential temperature effect between 2 and 8°C.
The accumulated thermal time from planting to the first flowering line and to the last flowering line were 951 to 1269 °Cday (representing 19 d) and 985 to 1286 °Cday (18 d) for R18xMo17 and N209xMo17, respectively, in 2010.
Lower expression of HvVRT2 and HvBM1 in the early flowering S42-IL107 (Ppd-H1) than in the late flowering line Scarlett (Ppd-H1) may thus reflect differences in development rather than direct effects of the mutation in Ppd-H1.
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A systematic search of exotic P. sativum germplasm [22] identified nine white flowered lines.
Of these 10 white flowered lines 7 carry the JI 1987 indel and three do not.
This G to A change was found only in white flowered lines while the other SNPs predicted to lead to amino acid changes were found in both colored and white flowered lines.
Sequence data groups the three uncharacterized white flowered lines with known wild types (Fig. S3C) so it is possible that these carry wild type A alleles.
Thus there was an absence of direct correlation between the level of FTa1 induction and flowering in the rapid flowering lines.
Grain samples could not be obtained from all 281 lines, especially low yielding and late flowering lines and a total of 252 lines have phenotypic data.
When grown outside without controlled vernalization, the earliest-flowering lines (Bd3-1, Bd21, and Bd21-3) flowered up to three months earlier than the latest flowering lines (e.g.Bd1-1 and Tek lines).
As mentioned above for plants grown outside, the stems of the earlier flowering lines were subjected to dramatically different environmental conditions than the stems of plants that flowered later.
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