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We used a mixed linear model (SAS, Procedure MIXED), with transgenic type (crop, F1 hybrid or first-generation backcross) as the fixed treatment effect, phenological traits of weeds (time to first flower, flowering duration and total number of flowers) as covariates, and block and treatment×block interaction as random effects.
In addition to traits related to vegetative phase change (last juvenile node and number of intermediate nodes), QTL analyses were also conducted for other traits that were likely to differentiate the ecotypes, namely: node to first flower; flowering precocity; anthesis time; tree height; tree shape; and juvenile internode length.
Likelihood ratio tests showed that a GLMM explained significantly more variation in the response for three traits (size at first flower, number of fruit and number of branches), whereas a GLMM and GLM explained similar proportions of the total variation in the response variable for the other four traits (days from bolting to first flower, flowering duration, seed size and seed number; Table 2).
Our hypothesis that water treatment would produce differences in time to first flower, flowering time length, and percent of plants flowering did not appear supported by this study.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com