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Arabidopsis transformation was performed according to flower dip protocol (Clough and Bent 1998).
Functional analysis that would further support the important role of WRKY28 in ICS1 gene expression were hampered by the lack of WRKY28 knock-out mutants or T-DNA insertion lines, while our efforts to achieve silencing of WRKY28 through Agrobacterium-mediated transformation with pHANNIBAL constructs via flower dip only resulted in seedlings that died shortly after germination.
Using a color suited for the center of the flower, dip a toothpick or dotting tool in nail polish.
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The Agrobacterium liquids (1 1 v/v) mixed with the constructs and pC(Delt GUS vector were transformed into Arabidopsis (Col-0) using the flower dipping method [ 49].
The recombinant plasmids were introduced into A. tumefaciens strain GV3101 and then transformed into Arabidopsis using the flower dipping method [ 36].
The binary construct 35S::MdCIbHLH1 was introduced into Agrobacterium tumefaciens GV3101, and Arabidopsis plants were transformed using the flower dipping method described by [ 36]. A. tumefaciens strain LBA4404 containing either the 35S::MdCIbHLH1:GFP or 35S::GFP binary construct was used to transform apple calluses.
The flowers, with or without a baby zucchini attached, are four for $1. Separate the flowers, dip them in a thin fritter batter (seasoned flour and water) and fry until golden.
Her greatest indulgences are frozen avocados and edible flowers dipped in raw chocolate.
The flowers, dipped in wax when fresh to make them "everlasting," that Victorian conceit, did not wither and die gracefully.
The prep for what he calls the "exploding flower pill pieces" involved the artist and his team photographing hundreds of flowers dipped in liquid nitrogen, then capturing them shattering like glass at the moment of impact.
Arabidopsis plants were transformed using the flower-dipping method.
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