The digest was passed through a C18 Sep-Pak to remove lipid-linked glycans, and the flow-through solution was processed as described above for glycoproteins.
The exo-mRNA fraction in the flow-through solution was concentrated by using a Micro-to-Midi Total RNA Purification System Kit.
In the case of loss in the volume of sucrose from the top of the chip due to flow-through, fresh sucrose solution was added to the sensing region.
The flow rate of each solution was ∼3 μl/s, corresponding to the speed of flow passage through the myofibril being ∼15 μm/ms.
Convergence to the optimal power flow solution is achieved through iterating between the bus-level sub-problems.
The columns were each rinsed with 50 μL of PBS and the combined flow-through solutions from all three steps were saved.
In another approach, the microreactor was preheated to 39 °C, and immunocomplex solution was flowed through the reactor, mixed, and washed.
The mixture was directly added to the 50 ml syringe attached to a glass syringe filter and the solution was allowed to flow through the filter by depressing plunger for 2 minute.
This case demonstrates a significantly higher concentration as most of the solution was directed to flow through the nanoholes, improving particle aggregation over the holes.
The solution was then collected by centrifugation (flow through, FT).
For subsequent IPs of flow-through solutions, 4 μL (0.8 μg) of anti-cystatin C antibody and 100 μL of MicroBeads were added to each flow-through sample, and the remaining steps were repeated as above.
