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The effects of 5HT-stimulation on the amount of intracellular SERT (flow through of the streptavidin beads) mirrored those of the cell surface SERT (Fig. 2C and D).
The total phosphovimentin and vimentin (as flow through of the streptavidin beads) was similar between truncated and mutant forms of transporter expressing CHO cells (Fig. 7E and F).
Lanes 3 and 5 (Fig 6B) are the flow through of the interaction reaction shown in lane 2 and 4 respectively.
Tetramers and higher oligomers were obtained when trigger-free S100B was mixed with the flow through fractions, but not the wash fractions, and there was a clear maximum of potency to trigger S100B oligomerization in fraction 33 representing the late flow through of the phenyl sepharose column.
The two "pressure chamber" models of AERs evaluated could not achieve flow through of the inoculated tubing therefore there was no bioburden reduction.
The three resulting cell fractions are i) peripheral non-infiltrating NK cells in the supernatant after centrifugation (P), ii) tumor cells in the flow through of the MACS column (F), and iii) infiltrated NK cells recovered after elution from the MACS column (E).
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Lane 5: Flow-through of the second Ni-NTA affinity column.
Although the concentrations of Fe and TOC changed during groundwater flow-through in the 09MI20 borehole, they were approximately constant (2.9 ± 0.4 μg/L and 1.3 ± 0.1 mg/L, respectively) during the flow-through of the groundwater in the 10MI26 borehole (Figure 6b,c).
Electrophoretic analysis of the proteins in the flow-through of the C18 phase showed the absence of hemoglobin and larger proteins/peptides, indicating that they had been captured by the C18 phase, preventing these polymerase chain reaction inhibitory proteins from reaching and binding to the subsequent matrix which would be used for DNA capture.
The flow-through of the centrifugal unit was reserved as the reference for SAXS.
The digest was loaded on a His-Trap HP column, and P-TEFb lacking the His tag eluted in the flow-through of the column, while undigested protein and TEV-protease eluted later in the imidazole gradient.
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