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The flow through material for each peptide was subsequently passed twice through chromatography columns containing beads conjugated to the matched phosphorylated cortactin peptide.
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The ε-prototoxin-containing flow-through material was collected and applied to a Ni-NTA affinity column (Qiagen).
The supernatants were then applied to ice-cold PBS conditioned MS-columns (Miltenyi Biotec) placed into a magnetic field, and the flow-through material was collected.
After digestion, the mixture was passed through a 250 μm pore size nylon sieve and the flow-through material passed through an 80 μm pore size nylon sieve.
A way to determine energy flows is through material flow determination, which is used as a material inventory of processes.
First, the high flow-through support material POROS-OH was activated with disuccinimidyl carbonate (DSC), and the VHH was bound in a random manner via amines located on the protein's surface.
Samples of the flow-through (unbound material) and elution were analysed by SDS/PAGE.
Retained and flowed through materials are quantified by using the advantage of the covalently linked fluorophore label.
A sufficiently strong electrical force can break free a large number of electrons from the atoms that form the structure of the solid so that a large current can flow through the material.
The viscosity contrast is not as high as in permeable flow through porous material.
Photothermal processing leads us to the modeling of heat flow through the material.
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