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For instance, in vitro experiments showed that the transfer of AQP2 via EVs isolated from kidney collecting duct cells was functional and increased the water flow of recipient cells [ 51].
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Hence we tested BMT recipients for the presence of antidonor antibodies early (1 and 2 weeks) and late (>3 months) after BMT through flow cytometric assessment of recipient serum binding to donor cells.
The purified CD4+ T cells, which we confirmed by flow cytometry were devoid of recipient APCs (data not shown), were subsequently stimulated ex vivo with irradiated donor spleen cells.
This corresponds to results from repeated PB flow cytometry analysis of recipients within the 7-month period where no eGFP-positive or CD45.2 donor cells were found (not shown).
As with aid, oil revenues and other unearned wealth, a flow of cash risks making recipients passive and dependent.
These features enable simultaneous stable gene delivery in a large fraction of recipient cells thus allowing flow cytometry or bead capture selection instead of drug selection [ 14].
Indeed, when assortative mating allows for long-distance filtered pollen flow, the shifts in the genetic values of recipient populations are strongly enhanced.
Technology, notably mobile phones and online banking, and competition between remittance agencies, make exchanging money between individuals ever easier, safer and cheaper.Even if some remittance money is "wasted" (in development terms) on consumer goods, the flow of cash boosts demand in recipient economies and supplies precious hard currency.
Verbs like "disseminate" and "tailor" are used more frequently in the latter frameworks than in the Yokohama Strategy, which further reinforces the notion of a one-way flow of knowledge from experts to recipient communities.
After 3 weeks, cells were isolated from the spleen and bone marrow of recipient mice and analyzed by flow cytometry.
Szulanski (1996) describes knowledge transfer as a process of dyadic exchanges between the source and the recipient through four stages: initiation and implementation, including (i) events that lead to the decision to transfer from the source to the recipient and (ii) the flow of knowledge; and ramp-up and integration that begin when the recipient starts utilizing the transferred knowledge.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com