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This microfluidic growth chamber allows growth only in one dimension resulting in a single layer of cells; however, cells are surrounded by a constant flow of medium.
This finding is not surprising since the flow of medium caused by perfusion rapidly dilutes the released cytokines in the entire volume (10 ml) of medium in the perfusion reservoir.
The interconnectivity of the porous structure of the scaffolds helps the flow of medium throughout the scaffold for even cell adhesion.
The disk was positioned eccentrically on the bar with an external flow of medium to increase the rate of solvent flow over the disk surface.
A 3D model was constructed to simulate the flow of medium through the outer wall of the gear tooth surface using computational fluid dynamics (CFD) approach.
In the model, the motion of discrete mineral particles is obtained by DEM and the flow of medium (mixture of water, air and fine magnetites) phase by the traditional CFD.
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To assess the antimicrobial/antibiofilm activity of the new e-chem NO releasing catheters, the total adhered viable bacteria on their surfaces after exposure to a flowing stream of medium containing the bacteria for 3 d was determined.
The 0.02 ml/hr flow rate of medium corresponds to an average flow velocity of ~1 mm/s within each channel.
The total vessel density (mm/mm), perfused vessel density (mm/mm), De Backer score (1/mm), microcirculatory flow index of small vessels (MFIs) and microcirculatory flow index of medium vessels (MFIm) were obtained at baseline and after 24 and 36 hours.
The flow rate of medium through each lane was approximately 230 µL min−1, and flow cells were incubated at 30°C±2°C throughout the length of each experiment.
The bead bed was washed with 12 mL medium and the flow rate of medium through the column measured in triplicate.
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