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Exact(4)
When the flow nucleotide is not one of the candidates given by the current state, there is no incorporation transition.
One appropriate prior that we use is the geometric distribution:, where is the proportion of the current flow nucleotide w i in the genome.
Given a flow nucleotide, a state s can transition to at most two states, one where an incorporation occurs, denoted, and one where no incorporation occurs, denoted.
For example, when the state is, and the flow nucleotide is A, the next state can be either (if there is an incorporation event, A is now the only non-candidate) or (if there is no incorporation event, A is no longer a candidate).
Similar(56)
For example, if the flow nucleotides are two repetitions of the wash cycle 'ACGT', and the sequence itself is GCCT, then the flow-space representation is (0,0,1,0,0,2,0,1).
When the flowed nucleotide is complementary to the template strand in a well, the existing DNA strand in this well is extended with additional nucleotide(s) by a polymerase.
Even when the current nucleotide in the flow should be incorporated by all template clones, clones might not, by chance, incorporate it (for example, if no dNTP molecule is found in the physical vicinity of the template).
The cyclically flowed nucleotides and the corresponding flow values build the basis for not only base calling, but also per-base quality score calculation (integrated in Titanium output).
The 454 pyrosequencing technology is based on sequencing-by-synthesis and consists in the cyclic flowing of nucleotide reagents (repeatedly flowing T, A, C, G) over a PicoTiterPlate™.
With little evidence for recombination and gene flow, most nucleotide divergence between AcBoT and AcBoL must have accumulated through mutation.
We have shown that membrane bound ecto-NDPK acts extracellularly to contribute to the maintenance of vascular tone regulating blood flow via nucleotide receptor activation (Buxton et al, 2001).
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