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If we want to keep the number of necessary characterisation measurements within acceptable limits, we need to be confident that a flow instrument design reacts in a predictable and straightforward way to systematic influences.
In this study we utilize new developments in heat pulse theory, low-cost electronics, and 3D-printing to fabricate, calibrate, and field test an affordable research-grade sap flow instrument.
FeNo measurement was conducted using a NIOX-MINO (50 ml/min flow) instrument.
The arterial blood column diameter and centreline blood speed were measured using a Canon laser Doppler retinal blood flow instrument (CLBF 100; Canon, Tokyo, Japan) [ 8].
At each specific time point, equal volumes of 0.4 μM ternary complex and 2 μM programmed ribosomes were mixed and quenched with 40% formic acid using KinTek quench flow instrument at 20 °C.
From a flow instrument, constructed and validated by Chen, we selected eight items (Cronbach's α >0.7) used for assessing aspects of concentration (focusing, time distortion, loss of self-consciousness, and telepresence).
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The water permeability tests of polymer and lipid vesicles were conducted on an SF-300X stopped-flow instrument (KinTek Corp., PA) at 10 °C, where vesicles were rapidly exposed to a hypotonic osmolyte (10 mM Hepes and 0.01% (w/v) NaN3 at pH of 7).
The results indicate that there is an initial collapse of the protein in the deadtime of the stopped-flow instrument, corresponding to a regain of ∼60%% of the native signal, followed by three slower transients.
The inset photo is a black-and-white camera (CAM-B) image of a heat-flow instrument during dive 30 that is completely submerged in the bottom turbid layer.
The reaction was initiated by rapid mixing of the IC and TC in a quench-flow instrument.
The water permeability of RhCG proteoliposomes was measured with the carboxyfluorescein quenching method [42] using the SFM400 stopped-flow instrument (Bio-logic, Grenoble, France).
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