For cell sorting, total BM cells were stained with an anti-mouse c-Kit, followed by enrichment with anti-APC micro-beads (Miltenyi Biotec); the enriched cells were then stained with surface markers and sorted by flow cytometry (Influx, BD Biosciences) for further in vivo and in vitro investigations.
Acute inflammation is characterized by an increased blood flow, rapid influx and activation of immune cells, and the release of pro-inflammatory cytokines.
21 As a result, not only do the blood vessels adapt and become hyperpermeable, allowing for increased blood flow and influx of inflammatory cells, but lymphatic vessels also expand to contribute to characteristic flushing, erythema, and edema.
Therefore, the influx flow rate of each section is a function of the location and is defined by the well flowing pressure at the node.
Anklam (2005) proved that pressure in the well increases along the pipe moving from the hill to the toe region, and the influx flow rate decreases in the same direction.
In this study, an extended two-fluid model (TFM) describing the gas-liquid two-phase flow when gas influx occurs and an acoustic model describing sound propagation within section of marine riser are proposed.
The cell density of each culture was determined by counting DAPI-stained cells using a Cytopeia Influx flow cytometer.
Cells or no-cell controls were sorted directly into PCR-compatible microtiter plates using an Influx flow cytometer (Cytopeia, BD).
After trypsinization, cell pellets were resuspended in DAPI solution (2 mM CaCl2, 22 mM MgCl2 0.1 mg/mL BSA, 0.1% Nonidet P-40, 10 µg/mL DAPI, 10% DMSO) and run on an InFlux flow cytometer (Cytopeia).
