The percentage of antibody positive cells was analyzed by the FACSCalibur flow cytometry system.
Cells were analyzed on an LSRII flow cytometry system (BD Biosciences).
Cell sorting was performed on a FACS VantageSE or FACSAria flow cytometry system (BD Bioscience, Franklin Lakes, NJ, USA).
Here, a high throughput flow cytometry system developed by Dr. Sklar and his colleagues deserves a special attention [35].
FACS analysis was carried out on the FACScan Flow Cytometry System (Becton, Dickinson and Company, USA) using the CellQuest analyser program.
Viability was determined using the Guava EasyCyte Mini Flow Cytometry System, and the Guava ViaCount Reagent (Millipore; cat no. 4000-0040).
Five laboratories used the FACSCount (BD Biosciences) due to its robust performance on the WHO/ NHLS CDREQAS program across African sites [9]; the KAVI laboratory used the FACSCalibur flow cytometry system (BD Biosciences) for CD4 and CD8 counts.
More than 5×104 labeled cells were acquired and analyzed using an upgraded 5-color FACScan flow cytometry system (Cytek Development Inc., Fremont, USA) running CellQuest 3.3 software (BD).
Samples were resuspended in 0.5 ml PBS and stained with propidium iodide in the dark for 30 min, and the DNA contents were measured by fluorescence-activated cell sorting (FACS) on a Becton-Dickinson FACScan flow cytometry system.
Luminex's xMAP technology is the most advanced bead-based technology currently available; it involves a flow cytometry system that can handle 96-well microtiter plates and is equipped with advanced digital signal processing hardware and software.
After 3 days, immediately prior to the start of the antibiotic treatment, embryos were run through the COPAS flow cytometry system to determine the total level of red fluorescence, representative of bacterial load.
