Exact(25)
In these cases, after carefully checking histograms from the usual flow cytometry protocol, Accellix values appeared to be in error.
The flow cytometry protocol was adapted from previously described methods [25], [26].
Using the flow cytometry protocol, separation was greatly improved and clumped cells could be excluded from analysis.
We optimized a multiparametric flow cytometry protocol to analyze STAT5 phosphorylation upon cytokine stimulation in stem and progenitor cell compartments at a single-cell level.
Assays ran for 4 hours before collecting and quantifying the cells migrated into the bottom chamber using a fixed volume count flow cytometry protocol (Epics XL, Coulter).
We thus developed a 9-color flow cytometry protocol, in which we combined NK cell identification markers (positive and negative) with antibodies against 5 inhibitory NK cells receptors and against one effector function marker for degranulation, CD107a [30].
Similar(35)
Flow cytometry protocols are used for checking ploidy among genebank accessions and breeding materials.
Flow cytometry protocols designed to identify mouse eosinophils typically target Siglec F, an α-2,3-sialic acid binding transmembrane protein expressed universally on cells of this lineage.
In conclusion, our optimized multiparametric flow cytometry protocols allow the analysis of signal transduction at the single cell level in normal and leukemic stem and progenitor cells.
Flow cytometry protocols refined by Jackson et al. [37] and Cogswell et al. [38] were used to determine the success of triploidy induction in crosses whose eggs were treated with cytochalasin B. An initial screening was done on the larvae of each cross [38] to validate the success of the induction.
This potential aspect now deserves to be validated in multicentric clinical studies using standardized flow cytometry protocols.
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