Sentence examples for flow cytometry instrument from inspiring English sources
Then, the apoptotic and necrotic features in the drug-treated C. albicans cells were evaluated using fluorescence microscopy and flow cytometry instrument detection, respectively.
Using the well-established MB49 murine TCC line in culture, we found 69.6 % of cells to be labeled with our PEG Gd2O3 TRITC MSN, with most cells fluorescing between 2 and 3 orders of magnitude above the detection threshold for the flow cytometry instrument.
The flow cytometry instrument and procedures have been specified elsewhere [22], [34].
Flow cytometry was carried out on an 8-color flow cytometry instrument CYANADP Wistar Institutee, Philadelphia, PA) with Cell-Quest software (Becton-Dickinson, San Jose, CA) used in accordance with the manufacturer's instructions.
Relative counts of CD4+ T cells were determined in EDTA-treated peripheral blood by a direct immunofluorescence method [17] using a combination of anti-CD3 peridin chlorophyll protein (PerCP), anti-CD4 fluorescein isothiocyanate (FITC) and anti-CD8 phycoerythrin (PE) on a mobile volumetric flow cytometry instrument, the Cyflow SL blue (Partec GmbH, Münster, Germany).
Around 4000 events were collected per sample using the ImageStreamX imaging flow cytometry instrument and Inspire software (Amnis, Seatle, USA).
Flow cytometry instruments enable numerous samples to be run in batch automation for screening various potential drugs and doses with little extra effort.
These proficiency panels revealed a high variability between the results of different laboratories conducting MHC multimer staining, arguing for harmonization guidelines and improved quality assurance of both flow cytometry instruments and reagents.
After the experimental incubation, the cells were rapidly cooled to 4°C, diluted according to their density (from no dilution at all, up to 1 100 dilution), to avoid coincidence in the flow cytometry instruments [ 69, 70], and transferred into pre-cooled u-shaped 96-wells plates (Costar, Corning, USA) for the automated flow cytometry analysis.
Using flow cytometry (FACSort instrument and analysis software; ModFit LT), the cells were analyzed with regard to cell cycle distribution and apoptosis.
The frequency of CD4+CD28null T cells in peripheral blood and synovial fluid was analysed by four-colour flow cytometry (FACSCalibur instrument; Becton Dickinson Immunocytometry Systems, San Jose, CA, USA) in peripheral blood mononuclear cells and synovial fluid mononuclear cells after Ficoll separation (Ficoll-Paque Plus; GE Healthcare Biosciences AB, Uppsala, Sweden).
